PlexSeq Mid-Density (MD)
PlexSeq MD - Proven Efficiency
The PlexSeq Mid-Density platform bridges the gap between single-plex and high-density arrays, delivering high-quality data, fast turnaround and lower costs.
As a core technology at AgriPlex, this amplicon-based sequencing method is highly versatile for assaying 25 to 5,000 SNPs. With multiplexing, you can simultaneously genotype all SNPs across thousands of samples, saving time and money. Our innovative PlexCall software then analyzes allele frequencies and compiles SNP calls into a concise report. Assays built on this platform have supported tens of millions of samples in multiple labs and across many species.
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Workflow Advantages Streamline In-House Lab Operations
A custom solution built on this PlexSeq platform will save time, cut costs and increase throughput. Advantages include:
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Needs less than 4 ng of DNA per reaction well
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Works with crude DNA
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More efficient than bead-based extraction
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Conveniently fits your workflow
Busy Lab Managers Rely on AgriPlex Reagent Expertise
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Expert Support – Use our skills to customize and validate your assay design
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Efficient DNA Extraction – Save time, money and labor
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Primary PCR – Multiplex up to 5,000 SNPs
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Secondary PCR – No complicated library preparation or ligation steps
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Pool Bar-Coded Amplicons – One clean up and DNA quantification
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Next-Generation Sequencing – Over 55K unique CDI and 35K UDI barcodes are available
PlexCall – Compiles Calls and Reads in Convenient Report
Our innovative PlexCall software analyzes allele frequency and compiles your SNP calls into a concise set of results. Each report is tailored to your specific panel markers. The result is a fast and convenient way to review your data that streamlines the final step in your workflow.
Ideal Solution for New Product Development
​PlexSeq MD is an efficient solution in development and breeding stages for new products. Unlike fixed arrays, which can take a year to change, a custom SNP list can be integrated into the PlexSeq MD platform in as little as 6 to 8 weeks. This versatility can accelerate R&D in rapidly changing markets.
Research and Breeding Applications
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Quantitative Trait Loci (QTL) Analysis & Genetic Mapping
Genotype markers throughout the genome of parental lines and progeny to identify and map QTLs for your desired traits. ​
Trait Profiling
Create your own panel of trait-associated markers and profit from our ability to assay an unlimited number of samples to screen any germplasm for your traits of interest.​
Marker-Assisted Selection, Genomic Selection & Backcrossing
Simultaneously genotype background markers together with your trait markers to accurately estimate background recovery and recapture valuable genes from the recipient line. ​
Genetic Purity
& Varietal ID
In upstream breeding stages, confirm the genetic makeup of your lines or closely related germplasm with higher resolution. (For higher throughput QA QC, consider the PlexSeq Low Density solution.)​
